BIOT125 Biotechnology Methods and Techniques
Department of Science, Technology, Engineering & Mathematics: Biotechnology
- I. Course Number and Title
- BIOT125 Biotechnology Methods and Techniques
- II. Number of Credits
- 4 credits
- III. Number of Instructional Minutes
- 4500
- IV. Prerequisites
- CHEM121
- Corequisites
- CHEM121
- V. Other Pertinent Information
- None
- VI. Catalog Course Description
- This course is an introduction to common practices and methodologies of Biotechnology. Students receive hands-on experience using and maintaining laboratory instruments. Laboratory procedures include: solution preparation, aseptic technique, protein separations and assays, DNA electrophoresis, and Polymerase Chain Reaction (PCR). Lectures support the laboratory activities.
- VII. Required Course Content and Direction
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Course Learning Goals
Students will:
- recognize and employ laboratory safety practices when using biological and chemical materials;
- prepare and manage accurate laboratory notebooks and instrument logs;
- operate, calibrate, and maintain standard equipment found in a biotechnology laboratory;
- define and perform common laboratory measurements using micropippetors and volumetric apparati;
- explain and perform protein detection and quantification assays; and
- practice basic DNA procedures, including restriction digestion and gel electrophoresis.
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Planned Sequence of Topics and/or Learning Activities
- introduction to the Biotechnology Lab
- laboratory safety and documentation
- laboratory measurements and math
- solution and media preparation
- aseptic techniques
- spectrophotometry and graphing
- filtration and centrifugation
- protein detection and quantification
- DNA digestion and electrophoresis
- Polymerase Chain Reaction
- Student Project Presentations
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Assessment Methods for Course Learning Goals
Student progress and achievement is assessed through lab reports, project presentations, worksheets, take-home research assignments, and in-class exams. -
Reference, Resource, or Learning Materials to be used by Student:
Students use educational resources as approved by the department and specified in the individual instructor's syllabus.
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Review/Approval Date - 4/09; New Core 8/2015